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    Colorimetric nanoprobe-based detection of Helicobacter pylori using direct targeting of nucleic acids

  • Asma Moradi,1 Hamidreza Mollasalehi,2,* Dariush Minai-Tehrani,3
    1. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University,
    2. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University,
    3. Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University,


  • Introduction: Helicobacter pylori is known as a type 1 carcinogen and causes gastritis, stomach ulcers, gastric cancer, and mucosa-associated lymphoid tissue (MALT). H. pylori infection is mainly diagnosed by non-invasive methods such as urea breath tests, or by invasive methods such as endoscopy. Due to the importance of rapid diagnosis of H. pylori, we developed a nanoprobe-based method for direct detection of the target in the cells.
  • Methods: A specific target region was selected, and complementary probes were designed. The probes were used to functionalize gold nanoparticles. The genomic DNA contents of the samples were retrieved by the boiling method. The nucleic acid content of the cells was used for the further detection procedure. After a denaturation and annealing stage, magnesium chloride salt was used for induction in the colorimetric assay.
  • Results: The results show that the designed Au-nanoprobes were capable of attaching to the target region specifically among ten different bacteria, providing a specific colorimetric detection approach in less than 10 min. Moreover, the optimized concentration of the salt yields an optimum color differentiation between the positive and the negative samples. In positive samples, the nanoparticles stayed dispersed (red) due to a successful hybridization while in negative samples, aggregation occurred due to lack of a complementary sequence resulting in a blue shift.
  • Conclusion: Non-crosslinking gold nanoprobe method could be used as a simple, rapid, affordable method to nanodiagnosis of H. pylori and help restraining the spread of H. pylori infection.
  • Keywords: Helicobacter pylori-Gold nanobiosensor-Nanodiagnosis