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    Isolation of bacteriocin-like inhibitory substances (BLISs)-producing Staphylococcus epidermidis from different Iranian clinical sources

  • Mehran Amir Javid,1,* Mohammad Hosein Sadeghi Zali,2
    1. Department of Biotechnology, Urmia branch, Islamic Azad University, Urmia, Iran.
    2. Department of Bacteriology, Veterinary Faculty, Islamic Azad University, Urmia branch, Urmia, Iran.


  • Introduction: The emergence of pan-drug resistant strains (PDR) of bacteria has led to renewed efforts to identify alternative agents, such as bacteriocins and bacteriocin-like inhibitory substances (BLISs). In recent years, Bacteriocins have been recognized as natural preservatives in food and drug industries, also, nowadays, they are used as substitutes of chemical antibiotics for treatment of infections.
  • Methods: In a cross-sectional study, 150 Staphylococcus epidermidis strains were isolated from different Iranian clinical sources (patients with burns, wound and skin infections, UTI, diarrhea, eye infections, human skin wounds, and soft tissue infections) during a 3-month period. isolates were investigated by Spot test and Well diffusion test for their ability to produce compounds exhibiting antibacterial activity. BLISs production was induced by mitomycin C (2 g/mL) in S. epidermidis.
  • Results: 23 isolated from the S. epidermidis had potent activity against Bacillus cereus, Listeria monocytogenes, Klebsiella spp., Staphylococcus aureus, P. aeruginosa, Proteus spp. and Vibrio parahaemolyticus. The BLIS was heat-stable (up to 100°C for 15 min) and active within a pH range of 3 - 9. High yields of pyocin at 35 – 37°C, which declined sharply at temperatures above 37°C were obtained from all the producing strains. BLIS production started in the mid-exponential phase of growth, and the maximum level occurred in the late-stationary phase after 30 hours of incubation at 37°C.
  • Conclusion: The produced bacteriocin had a wide antibacterial activity spectrum against the gram-positive and negative bacteria, in particular pathogenic bacteria, also was resistant against heat and pH ranges. As a result, use of bacteriocin in food and drug industry, as animal feed, and as a substitute for chemical antibiotics is recommended.
  • Keywords: Bacteriocin, Staphylococcus epidermidis, Antibacterial, Drug-resistant