A new method for measuring Escherichia coli
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fatemeh ahmadzadeh,1,* ali saboonpaz,2
1. Afaq Higher Education Institute of Urmia
2. Afaq Higher Education Institute of Urmia
- Introduction: Nanotechnology is a knowledge and technology that has attracted a lot of attention recently. This technology, which is the new approach of all disciplines, has the ability to produce materials, new instruments by manipulating the atomic and molecular levels.
Nano science gives a different attitude about the emergence of new phenomena. In this view, a number of simple interactions between the components of the system, a new property in the whole system, different from the properties of its components, is revealed, which has been largely observed in computer simulations.
Nano science gives a different attitude about the emergence of new phenomena. In this view, a number of simple interactions between the components of the system, a new property in the whole system, different from the properties of its components, is revealed, which has been largely observed in computer simulations.
- Methods: First, 100 ml of iron dissolving two sulfate and 100 ml of three sulfate iron solution should be prepared and after mixing them together into a human, humans and their contents should be placed on heat and then 20 ml ammonia should be poured into the burt and droplets should be added to the solution, and in addition to adding ammonia, the container containing the solution should be arami.
After 20 ml of ammonia is added to the solution, smooth the final solution by filter paper and wait 24 hours until the water that comes with the nanoparticles evaporates and the nanoparticles remain powdered on the filter paper.
Then, add the fluorescent colored material (Violet crystal) to the water solution and nanoparticle to absorb the nanoparticle surface and then add the anticorbic to absorb the nanoparticle.
Then put the solution, which has the above contents, into a box and create two holes in the box and from one of the holes, uv rays are irradiation into the solution placed in the box.
- Results: After producing magnetic iron nano particles and adding fluorescent (violet crystal) and anti core to the solution, the solution contains the above contents into the box and by irradiation of ultraviolet radiation into the solution, mercury vapor lamp produces a wave length in the ultraviolet region. The bacteria binds to its own anticore and by binding to magnetic nano particles and fluorescence materials, it is produced in the light of the glow.
In the absence of fluorescence bacteria, there are no light spots in the presence of mercury vapor lamps, but in the presence of bacteria that are adsorption of nano particles containing anti core and pigment, the intensity of fluorescence increases and is seen as light spots.
- Conclusion: Due to the pathogenicity of bacteria and the risk of bacterial infections, rapid measurement of bacteria in disasters such as earthquakes, floods, etc. It becomes necessary. However, using this method without special facilities in the shortest time and with the lowest possible costs, it measured even very small amounts of bacteria that are not measured by standard methods.
- Keywords: Magnetic nano particles, coli bacille, anticorbic, fluorescence, ultraviolet radiation
