Antibacterial, antioxidant and anticancer effects of the hydroalcoholic extract of Eucalyptus Globulus leaf growing in the north of Iran
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Amir Arasteh,1 Morteza Karimpour,2,*
1. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
2. Department of Biology, Rasht Branch, Islamic Azad University, Rasht, Iran
- Introduction: Eucalyptus Globulus is the kind of Myrtaceae trees that is originally from Australia and forms a dense forest. Its height is from 30 mm to 145 m. and its stem diameter reaches 25 m. This research aims at studying the antimicrobial, antioxidant and anticancer effects of the hydroalcoholic extract of Eucalyptus Globulus leaf.
- Methods: First, Eucalyptus Globulus leaves were powdered, and the hydroalcoholic extract was prepared with using ethanol solvent 70%. The antimicrobial effect of this extract was examined on Staphylococcus aureus, Escherichia coli, Listeria monocytogenes, and Salmonella typhimurium bacteria using antibiogram method. In addition, MIC and MBC values of Eucalyptus Globulus hydroalcoholic extract were determined. The antioxidant effect was examined using DPPH method. To test the anticancer effect of the extract, MTT method on gastric cancer cell line (AGS) was used.
- Results: The diameter of the inhibition zone of Eucalyptus Globulus hydroalcoholic extract in well method on Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Salmonella typhimurium was 12, 22, 28, and 15 mm, respectively, In addition, MIC and MBC in Escherichia coli and Staphylococcus aureus were 0.625 and 1.25 mg/ml, in Listeria monocytogenes was 1.25 and 5 mg/ml, and in Salmonella typhimurium was determined 5 and 10 mg/ml, respectively. The maximum antioxidant activity was recorded in 9 mg/ml extract concentration. Cells viability in MTT test was obtained 79.9% in the presence of extract.
- Conclusion: Eucalyptus Globulus extract has antimicrobial, antioxidant and anticancer properties and can be a proper alternative for infection treatment, reducing cancer growth and as an alternative to chemical preservatives in the food industry.
- Keywords: Eucalyptus Globulus, Hydroalcoholic extract, antimicrobial, antioxidant, anticancer
