Use of a consortium of halophilic bacteria isolated from the Salt Lake of Howz-E Soltan of Iran to increase the efficiency of Hydrolysis enzymes
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Mastoore Asadbeygi,1 Ali Mohammadi,2,* Mohammad Reza Soudi,3
1. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
2. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
3. Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran
- Introduction: Hypersaline lakes, with salinity, ranges at or near saturation are extreme environments; yet, they often maintain remarkably high microbial cell densities and are biologically very productive ecosystems. To adapt to saline conditions, bacteria have developed various strategies to maintain
cell structure and function. Studies of such bacteria are of great importance, as they may produce compounds of industrial interest, such as extracellular, hydrolytic enzymes that have diverse potential usage in biomedical science and chemical industries. Halophiles are an excellent source of such enzymes which are not only salt-tolerant, but also may be active at high temperature and pH values.
- Methods: Bacteria were isolated from the hypersaline site, in a solid medium supplemented with various salt concentrations ranging from 1 to 20 g/L. Physical and chemical characteristics of samples from the isolation site were determined to suggest eventual correlations with the occurrence of the halophilic bacteria. Assays on enzymatic activities were performed in submerged cultures in the presence of various salt concentrations and appropriate substrates.
- Results: As a result, the growth of the strains in the medium containing 1to20% (w/v) NaCl determined that the majority of the isolates were moderately halophile. The results represented that H2-5 and H2-6, have CMCase activity and increase together as a consortium. The bacteria cultures for evaluated for their antibiogram for diversity between halophiles and archaebacteria and it has shown that they are halophiles. Phylogenic tree analysis also showed that two strains of H2-5 and H2-6 belong to Metabacillus halosaccharovorans.
- Conclusion: Together, these two isolates showed high hydrolytic activity relative to separate activity and had good results, and the two, which are highly halophilic, have excellent biotechnological applications due to their ability to produce different hydrolysis. Highly halophilic bacteria showed higher potential for the production of consortium enzymes compared to moderate halophiles.
- Keywords: Halophiles, Hydrolysis, Isolation, Consortium
