Identification of mutual altered gene expression in 5 types of cancer as a target gene and potential oncogene
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Niloufar khayamnikouei,1 Zahra yousefian,2,*
1. Department of cellular and molecular biology, Faculty of Advanced Science and Technology, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
2. Department of cellular and molecular biology, Faculty of Life Sciences,North Tehran Branch, Islamic Azad University, Tehran, Iran
- Introduction: Studies have shown that cancer in developed and developing countries is the first and second leading cause of death respectively. Some factors have an important role in cancer development and lead to metastasis by destroying normal cells. In the current study, we identified commonalities in gene expression among 5 types of cancer, such as rectum, colon, breast, liver, and lung.
- Methods: In our study, we utilized TCGA data to identify expression profiling of the genes among the 5 cancers. For this purpose oncoDB (http://oncodb.org/) was used and raw gene-level read counts were normalized using the transcripts per million (TPM) method. For comparative analysis of cancer samples and normal samples, we obtained gene expression from The Cancer Genome Atlas (TCGA) for all 5 types of cancer. Moreover, we used an interactivevenn (http://www.interactivenn.net/) to identify the commonality of genes associated with these 5 types of cancers.
- Results: The results of differential expression analysis between cancer samples and normal samples showed some altered gene expression. A p-value of <0.05 and |log2FC |>1 was regarded as statistically significant. 19 mutual genes from the 5 types of cancers were selected and 15 of 19 genes were overexpressed and led to tumorigenesis. Furthermore, we proposed that the majority of these genes overexpression induce tumor proliferation in tumor cells by regulating cell-cycle at the G2/M phase. Genes were such as MMP11, CTHRC1, SPP1, TRIP13, ANLN, IQGAP3, ORC6, RECQL4, UBE2C, TPX2, TROAP, NUF2, MYBL2, CENPF and CDC6.
- Conclusion: Upon evaluation, our results indicate that there are 15 mutual genes associated with rectum, colon, breast, liver, and lung cancers and demonstrated a significant correlation with the expression of genes and progression of cancer. We can consider the overexpression of these 15 genes as an effective factor in the transformation of normal tissue into cancer tissue. Furthermore, these known genes not only have a special role in the progression of cancer but can also be useful for targeted therapy.
- Keywords: Pan-cancer analysis,Cancer, TCGA, Gene expression, Oncogene
