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دانلود کتابچه

    Effect of various preparations of gingiva Extra cellular matrix on the surface attachment of Streptococcus mutans

  • Fatemeh Sadat Hosseinynam,1,* Hadi Maleki,2
    1. Department of Microbiology and Microbial Biotechnology, Faculty of Life sciences and Biotechnology, Shahid Beheshti University
    2. Department of Microbiology and Microbial Biotechnology, Faculty of Life sciences and Biotechnology, Shahid Beheshti University


  • Introduction: Investigating the biological phenomena in an In Vitro condition necessitates providing a test condition as close to the physiological state as possible. The surface properties of the cultivation chamber have a significant impact on the successful attachment and the subsequent proliferation of cells. Previous studies have proved that the extracellular matrix (ECM) proteins remarkably affect cellular adhesion and colonization. The potential of bacteria in adhesion and biofilm formation in the oral cavity is expected to be affected too by the ECM composition of the target tissue. The greater similarity to the target tissue, the more real results in the in vitro investigations on the oral cavity biofilms. Consequently, coating the cell culture plates and flasks with the related proteinaceous preparations seems reasonable.
  • Methods: In the present study, Streptococcus mutans ATCC 35668 was used as the most studied model organism in oral cavity-related investigations on biofilm formation, and its adhesion potential was compared in cell culture plates coated with various concentrations of pepsin digested ECM of gingivae, pronase E-digested ECM of gingivae, and the collagen A protein. The adhesion degree was determined by performing the MTT assay as a colorimetric method with specificity in quantifying the viable cells.
  • Results: Results showed that the number of viable adhered bacteria was higher when the pronase E-digested ECM was used as the surface coat in comparison to the other coatings.
  • Conclusion: It seems that all proteinaceous components of ECM together had a better performance in supporting the S. mutans compared to the collagen A alone. Also, the degree of enzymatic digestion and the profile of the resulting peptides might cause a difference in the adhesion efficiency.
  • Keywords: bacterial adhesion, culture surface coating, Streptococcus mutans, collagen A, extracellular matrix