Evaluation of the bacteria with transovarial transmission in Anopheles stephensi (mysorensis strain) for determining new candidates for paratransgenesis
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Mehrnaz Mehrvarz,1 Abbasali Raz,2,* Navid Dinparast Djadid,3 Maryam montazeri,4
2. Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran.
3. Malaria and Vector Research Group (MVRG), Biotechnology Research Center (BRC), Pasteur Institute of Iran, Tehran, Iran.
4. Department of Advenced Science and Technology, Faculty of Advanced Science and Technology, Tehran Medical sciences, Islamic Azad University, Tehran, Iran.
- Introduction: Malaria is one of the most important parasitic diseases throughout the world. The Anopheles stephensi is the main vector of malaria in Iran. WHO suggested different strategies for combatting against malaria. paratransgenesis is one of the effective solutions for developing the Global Malaria Eradication Programme. In paratransgenesis, symbiont microorganisms are manipulating genetically for blocking the parasite life-cycle, affecting the fitness and longevity of vectors. In this study, by sterilizing eggs of Anopheles stephensi and removing surface bacteria, vertically transmitted bacteria were identified in Anopheles stephensi mysorensis strain using the culture-dependent method.
- Methods: An. stephensi eggs were collected from the insectary in Pasteur Institute of Iran where they were raised. Sterilization was performed by the Brundage method for obtaining the sterile seven generation. The suspension was prepared from the sterile eggs and it was inoculated into the enrichment mediums. Then, dissimilar morphological colonies were selected for performing the differential biochemical tests. Next, PCR was performed by 16s rRNA gene specific primers on isolated clones and finally sequenced.
- Results: The results of this study demonstrated that the eggs of microbiota of An. stephensi mosquito were gram negative and gram positive bacteria which include: Serrratia marcescens and Asaia .
- Conclusion: According to the fact that the paratransgenic technique is an effective method for eradication malaria so, introducing the suitable candidates is essential for developing this technique. Therefore, in this study, the best bacteria candidates in An. stephensi mosquito eggs that have specific features like simple transformation capacity, and transovarial transmission were identified and provided the fundamental data for developing paratransgenic technique.
- Keywords: Paratransgenesis, Malaria, Anopheles stephensi.
