The association between increased expression of PRDX4 and MRPS23 genes with BMI, sex and stage of disease in colorectal cancer (CRC)
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Alireza Ahmadi,1,* Abedeh Rezaei,2 Fatemeh Sadat Seyed Varjavi,3 Kosar Taleb,4
1. Department of Animal Biology, Tabriz, Iran *4 authors contributed in this study equally
2. Department of Animal Biology, Tabriz, Iran
3. Department of Animal Biology, Tabriz, Iran
4. Department of Animal Biology, Tabriz, Iran
- Introduction: CRC is currently the third leading cause of cancer-related deaths and its incidence is increasing. According to studies, BMI and gender of patients can be mentioned as effective factors in the incidence of CRC .In this study, the genes with increased expression in CRC and its relationship with gender and BMI of patients as well as the stage of the disease were identified.
- Methods: CRC risk factors were studied through PubMed and Google Scholar databases and BMI and gender were considered as factors in this study. The Cancer Genome Atlas Program (TCGA) data were used to identify the altered genes expressed in CRC and their relationship with BMI and disease stage as well as the gender of patients. For this purpose, Oncodb database was used and gene expression was normalized by TPM method. At first, the genes that were more expressed in cancer samples compared to normal samples were identified. Common genes between the three factors, (BMI, stage and gender) were obtained using the Interactivenn site. Also, BMI above 29.9 were considered as samples of obese people, between 25 and 29.9 as overweight people, between 25 and 18.5 as normal people and less than 18.5 as underweight people. It should be noted that data classification and review was done with EXCEL 2019 software.
- Results: The difference in expression between cancerous and normal samples showed that 1421 genes had significant increase (| logFC |> 1, p-value <= 0.05). Also, the results of the study of the relationship between the expression of these genes with BMI, patients 'sex and disease stage, showed that PRDX4 gene expression with BMI (ANOVA-pvalue = 2.7e-03), patients' sex (ANOVA-pvalue = 4.9e-02) And disease stage (ANOVA-pvalue = 2.6e-03) were significantly correlated. MRPS23 gene expression was also significantly associated with BMI (ANOVA-pvalue = 3.9e-02), patient gender (ANOVA-pvalue = 3.3e-02) and disease stage (ANOVA-pvalue = 2.6e- 02). . PRDX4 gene expression was higher in men and obese individuals as well as patients in stage 1 disease than other groups. Also, the expression of MRPS23 gene was higher in men and obese individuals and patients in stage 2 of the disease than other groups.
- Conclusion: The results showed that the expression of PRDX4 and MRPS23 genes increased in CRC and this increase was higher in men and obese individuals than other groups. Since PRDX4 gene expression was higher in stage 1 disease, this gene is involved in the onset of the disease and can be a good candidate in the treatment of men with CRC with a BMI greater than 29.9 who are in the early stages of the disease. MRPS23 gene expression was also higher in stage 2 of the disease than others. As a result, this gene can be a good candidate in the treatment of men with CRC with a BMI above 29.9 who are in the early stages and stage 2 of the disease.
- Keywords: Gene expression, The Cancer Genome Atlas(TCGA), Colorectal cancer (CRC), Oncogene, BMI
