MDC1-AS1 lncRNA expression in glioblastoma
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Mansoureh Shabani,1,*
1. Department of Genetics, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University
- Introduction: Background and purpose:
Glioblastoma is one of the most malignant and common brain tumors, accounting for about half of all gliomas. Glioblastoma is a tumor of the central nervous system (CNS) that originates in the glial tissue of the brain.
The aim of this study was to investigate the changes in the expression of lncRNA 1 MDC1-AS as a possible biomarker in glioma.
- Methods: For this purpose, after reviewing the medical records of patients at Imam Hossein Hospital in Tehran, obtaining written consent from patients and code of ethics in Imam Hossein Hospital was performed. Paraffin blocks including brain tumor biopsy from 1394 to 1396 were collected and by The pathologist was confirmed and the degree was determined. In this project, 50 samples of grade 1 and 2 and also 50 samples of grade 3 and 4 were examined. For all tissue samples donated by patients, RNA extraction and cDNA synthesis were performed and then a specific primer and probe were designed and the expression level of MDC1-AS gene 1 using the technique Real-time PCR was evaluated. The mean age of the subjects was 43.70 16 16.416 years. Statistical analysis was performed descriptively and analytically using SPSS Ver20 software and unpaired Sample Student T Test was used to compare the amount. Expression of this gene was used in lower grade tumor tissue and higher grade tumor tissue.
- Results: The results showed that tumor samples with grade three and four had 0.202 times more expression (fold change) than tumor samples with grade one and two. And this distance is statistically significant
- Conclusion: The results of quantitative study showed that MDC1-AS1 gene expression decreased significantly with increasing disease degree (with P-value = 0.0001).
- Keywords: Glioblastoma, lncRNA, 1 MDC1-AS1
