The effect of NaCl and salicylic acid on total phenolic contents in suspension culture of Nitraria schoberi
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Dana Rafiee,1,* Nasim Zarinpanjeh,2 Mohammad Ali Ebrahimi,3 Nasrin Qavami,4
1. Department of Agricultural Biotechnology, Payam Noor University, Tehran, Iran
2. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
3. Department of Agricultural Biotechnology, Payam Noor University, Tehran, Iran
4. Medicinal Plants Research Center, Institute of Medicinal Plants, ACECR, Karaj, Iran
- Introduction: Nitraria schoberi is a medicinal plant with antioxidant, antifungal, antimicrobial, anti-inflammatory, and anti-viral properties. This plant is also tolerant to salinity and drought. Cell suspension culture is one of the most ideal methods for producing secondary metabolites, but they seldom produce sufficient amounts of the required secondary metabolites without proper elicitors. Elicitors are biotic or abiotic molecules that induce a signal across plant cells which, in turn, stimulate secondary metabolic pathways and downstream transcription factors in response to the external stimuli thus resulting in the production of secondary metabolites. The main aim of this study was to perform elicitation in cell suspension culture of N. schoberi to detect the production of total phenolic contents.
- Methods: In order to achieve that goal, hypocotyls and cotyledonary leaves of N. schoberi as explants were cultured in different callus induction media. At the next step, the calli belonging to the best callus induction medium were transferred to different cell suspension cultures containing elicitors including Sodium Chloride (NaCl) and Salicylic acid (SA) alone or in combination with each other. Total phenolic contents in treatments were measured using a spectrophotometer (absorbance at 765 nm and 430 nm, respectively).
- Results: Although callus induction was observed in all treatments, the maximum fresh weight (7.6 g) and dry weight (3.7 g) were obtained in MS medium supplemented with 2, 4-dichrolophenoxyacetic acid (2,4- D) and 1-naphthalene acetic acid (NAA) both at 0.5 mg/L. The highest total phenolic content (62.2 mg/g) was detected in suspension culture containing 100 mµ NaCl and 50 Mµ SA.
- Conclusion: . In conclusion, elicitation in suspension culture of N. schoberi can be an effective method for increasing the production of secondary metabolites (phenolic compounds).
- Keywords: Elicitor, Medicinal plants, Secondary metabolites, Suspension culture
