Evaluation of the Mother of Vinegar as a Potential Wound Dressing for Diabetic Wounds and its Antibacterial Properties
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Mahdi Tafteh,1 Sadaf Solati,2 Mitra Norouzi,3 Mojtaba Ranjbar,4,*
1. Microbial biotechnology Department, Amol University of Special Modern Technologies, Amol, Iran
2. Microbial biotechnology Department, Amol University of Special Modern Technologies, Amol, Iran
3. Biology Department, Shahid Beheshti University, Tehran, Iran
4. Microbial biotechnology Department, Amol University of Special Modern Technologies, Amol, Iran
- Introduction: Acetic acid bacteria are obligate aerobic microorganisms able to convert ethanol to acetic acid. One of the two methods for vinegar production is a traditional method. The traditional method is slow acetification carried out resulting in high-quality vinegar. It is a static method, the so-called surface culture method or Orleans method, where acetobacter is placed on the surface of the liquid, evolved into a biofilm in direct contact with oxygen. This biofilm, called “Mother of vinegar” is used as a wound dress in this study. In wounds caused by diabetes or burns, the rate of healing is low, and proper wound dressing provides the right conditions to prevent the wound from becoming infected and help improve wound healing. This wound dress with a bacterial source has nano-sized pores that prevent the passage of pathogens and contains acetic acid in its tissue, which can kill bacteria.
- Methods: A total of 30 male mice were kept in the pet for a week to adapt to the environment. Mice were divided into 4 groups: healthy, diabetic wound with cellulose wound dress, negative control, and positive control. After this period, we used Streptozotocin (STZ) to create a diabetic model. After 2 days, a blood glucose test was performed and all mice became diabetic. A wound 0.9 cm in diameter was then punctured in the back of the mouse and held in place by the wound holder. Picric acid was used to protect the wound. The results were observed and recorded on days 5, 10, and 15. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) assay were used for the antibacterial test of cellulose biofilm.
- Results: The results showed that the cellulose biofilm had better results than the positive control and negative control samples, so the wound healing percentage on days 5, 10, and 15 was 40%, 80%, and 98%, respectively, but the negative control on days 5, 10 And 15 had a 14%, 34%, and 70% improvement, respectively, which showed a beneficial result of this wound dressing. On days 5, 10, and 15 no infection was seen in any of the wounds. The results of antibacterial activity showed that the effect of wound dressing on gram-negative bacteria is more than gram-positive bacteria and is related to their wall type. Inhibitory concentrations for gram-negative and gram-positive bacteria are 0.2 and 0.3 grams, respectively, and lethal concentrations for gram-negative and gram-positive bacteria are 0.3 and 0.4 grams, respectively.
- Conclusion: Diabetic wound healing is one of the problems today that its repair requires a proper wound dressing. Problems with diabetic ulcers include the cost of treatment and amputation. Since in this study and similar studies, acetic acid heals wounds and due to the specific structure of this cellulose biofilm, it can be expected that this wound dressing is a suitable option for this treatment. In the results of the microbial test, this wound had the ability to inhibit and kill more gram-negative bacteria than gram-positive bacteria, and the reason for this difference, like other research, showed that differences in cell wall structure can achieve this result.
- Keywords: Wound dress, Diabetes wound, Acetobacter, Cellulose biofilm, Mother of vinegar
