Evaluation of the Effect of the Ni-Thiosemicarbazones complex on Expression Changes of CPEB2 gene in Acute lymphoblastic leukemia
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Arezoo Hassani,1 Mahnaz Eskandari,2 Golnaz Asaadi Tehrani,3,* Sina Mirza Ahmadi,4
1. Msc of Molecular Genetic Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
2. Msc of Molecular Genetic Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
3. Assistant professor of Molecular Genetics, Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
4. Assistant professor of Molecular Genetics Department of Genetics, Zanjan Branch, Islamic Azad University, Zanjan, Iran.
- Introduction: The aberrant proliferation and differentiation of a clonal population of lymphoid cells are involved in the pathogenesis of ALL. ALL has a poor prognosis, with only 10% of adult ALL patients surviving and 30% of pediatric ALL patients surviving. The complete remission rate in adult ALL is 30 percent to 40 percent in the first relapse and 20 to 25 percent in the second relapse with conventional chemotherapy. CPEB2 is increased in cancer tissues and increases the proliferation and migration of cancer cells of this kind. Thiosemicarbazones (TSCs) are a type of Schiff base made by combining thiosemicarbazone with an appropriate aldehyde or ketone. Chemists and biologists have been studying TSCs because of their diverse pharmacological properties. The application of these outstanding metal chelators against cancer is one of the promising areas in which they are being developed. The goal of this study was to see how Ni-thiosemicarbazone complexes affected the expression of the CPEB2 gene in a cell line that had been diagnosed with acute lymphoblastic leukemia.
- Methods: In the current research, two concentrations of Ni-Thiosemicarbazone complexes were prepared: 100.5µM and 104µM at 24hours. The Jurkat E6.1 cell line was purchased from Pasteur Institute and treated with prepared doses of the Thiosemicarbazones complexes Ni at 24 and 48 hours after cell passage. The expression changes of CPEB2 and GAPDH were studied using Real-Time PCR after RNA extraction and cDNA synthesis. Finally, Rest 2002 Software was used to analyze the data, and Excel was used to draw the diagrams.
- Results: The results of our findings showed that the expression of CPEB2 in comparison with the GAPDH housekeeping gene decreased after 24 hours of Ni-Thiosemicarbazone complexes treatment at concentration of 104 µM. According to the findings, changes in CPEB2 gene expression increased after 24 hours at a concentration of 100.5µM and 104µM decrease were statistically significant These changes included 100.5µM (0.019) and 104µM (0.984) at 24 hours, respectively. (P <0.001)
- Conclusion: According to the present study results, alternation in CPEB2 expression after treatment with Ni-Thiosemicarbazone complexes, at two concentration were effective in decrease of CPEB2 expression. Evidence showed that the Ni-Thiosemicarbazone complexes has positive potential and efficacy because the drug was ineffective in decreasing gene expression in two concentrations in 24 hours (p-value 0.001)
- Keywords: Ni-Thiosemicarbazones complexes, CPEB2, GAPDH, Acute lymphoblastic leukemia
